5 Simple Techniques For link naga26
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From the crystal composition with the D273N mutant of NagA, the side chain of His-143 is 3.0 Å far from the phosphonate oxygen from the mimic of your tetrahedral intermediate (21). This consequence indicates this team could facilitate the activation on the carbonyl group from the substrate along with an conversation While using the steel ion certain in the Lively internet site.
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Mutation of Y223 to your phenylalanine residue resulted inside a mutant with virtually the exact same catalytic activity since the wild sort enzyme and so this residue just isn't considerable for your binding of substrate in NagA.
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can bind up to at least one divalent cation with the activation of the hydrolytic water molecule. This water molecule is even more activated by general base catalysis from the abstraction of the proton by Asp-273.